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Therefore, intrinsic fluorescence-based studies are typically restricted to a limited number of aromatic residue-containing IDPs, which are usually characterized by surface location of Trp residues characterized by the red-shifted intrinsic fluorescence spectra with max at 340 353 nm (Permyakov and Uversky, 2010)

If you add 4ml to maintain the 2.5mg/ml concentration, you have twice the total volume to work through

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